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May 01,2005·The rDME-G gene was inserted in-frame with the initiator codon and a carboxy-terminal 6×-His tag-encoding sequence of the bacterial expression vector pQE60,under the control of an inducible PT5 promoter.This expression vector is depicted in Fig.3A.This vector was introduced into the E.coli host SG13009 (which harbors the pREP4 plasmid encoding the lacI repressor) and transformantsA repertoire of high-affinity monoclonal antibodies May 29,2015·Typhoid fever is a significant global health problem with highest burden on the developing world.The severity of typhoid is often underestimated,and currently available serological diagnostic assays are inadequate due to lack in requisite sensitivity and specificity.This underlines an absolute need to develop a reliable and accurate diagnostics that would benefit long-term disease
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May 04,2021·We report a vaccine platform to express vaccine antigens on the surface of genome-reduced bacteria to enhance vaccine immunogenicity.We demonstrate the utility of this vaccine platform by expressing the highly conserved fusion peptide (FP) of SARS-CoV-2 and porcine epidemic diarrhea virus on the surface of Escherichia coli to produce killed whole-cell bacterial vaccines.Memories and AdventuresHe came also of a famous athletic stock,and was a great Rugby forward himself,though rather handicapped by the Berserk fury with which he would play.He was up to international form,and his younger brother was reckoned by good judges to be about the best forward who ever donned the rose-embroidered jersey of England.
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