Jun 11,2019·Guinea pig polyclonal a-insulin antibody was produced in-house.For secondary anti- For secondary anti- bodies,goat a -mouse,goat a -rabbit,and donkey a -guinea pig antibodies(PDF) Synaptotagmin 11 Interacts with Components of the Purified recombinant proteins were incubated with INS-1E postnuclear supernatants and bound proteins were analyzed with appropriate antibodies guinea pig polyclonal anti-SND1 (1/1000),anti-PDIA6
Synaptotagmins are two C2 domain-containing transmembrane proteins.The function of calcium-sensitive members in the regulation of post-Golgi traffic has been well established whereas little is known about the calcium-insensitive isoforms(PDF) Translationally controlled tumour protein (TCTP) is Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells
Lane 1 Rabbit control IgG instead of (1g) instead of CSB-PA010824ESR2HU in Hela whole cell lysate.For western blotting,a HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2 CSB-PA010824ESR2HU (4g) + Hela whole cell lysate (500g) Lane 3 Hela whole cell lysate (20g)Altered expression of microRNAs in the response to ER Jan 01,2015·MicroRNAs,a class of small noncoding RNAs,play key roles in diverse biological and pathological processes.ER stress,resulting from the accumulation of unfolded or misfolded proteins in the ER lumen,is triggered by various physiological events and pathological insults.Here,using RNA deep sequencing analysis,we found that the expression of some microRNAs was altered in HeLa and
Jan 01,2015·RNA-seq was applied to detect the expression levels of miRNA in response to ER stress.HeLa and HEK293 cells were treated to induce ER stress with 300 nmol/L of TG,and the induction of the UPR to ER stress was verified by the cytoplasmic splicing of the xbp-1 mRNA .As expected,24 h of TG treatment induced ER stress exhibited as increased spliced xbp-1.Altered expression of microRNAs in the response to ERWestern blotting assay.The primary antibodies used were as follows mouse polyclonal anti-DGCR8 (1:500) (Sigma,St.Louis,USA),rabbit polyclonal anti-Drosha (1:500) (Sigma),rabbit polyclonal anti-Dicer (1:500) (Sigma),rabbit polyclonal anti-Exportin-5 (1:500) (Sigma),rabbit polyclonal anti-Ago2 (1:500) (Sigma),rabbit polyclonal
Feb 24,2017·Anti-ERp5 Antibody (G-5) is a mouse monoclonal IgG 2b ERp5 antibody,cited in 4 publications,provided at 200 µg/ml; specific for an epitope mapping between amino acids 15-45 near the N-terminus of ERp5 of human origin; Anti-ERp5 Antibody (G-5) is recommended for detection of ERp5 of mouse,rat and human origin by WB,IP,IF,IHC(P) and ELISA; also reactive with additional species Anti-GFAP Antibody,Rabbit Polyclonal,100139-RP02Anti-GFAP Rabbit Polyclonal Antibody (100139-RP02),manufactured by Sino Biological is validated in IHC-P.Custom antibody services and bulk production also available.To learn more comprehensive our antibody product information including immunogen,specificity,and more,you can read all details here.
More>> Anti-Integrin beta-3 Antibody is an antibody against Integrin beta-3 for use in WB,IP,IH(P).Less<< Anti-Integrin beta-3 Antibody MSDS (material safety data sheet) or SDS,CoA and CoQ,dossiers,brochures and other available documents.Anti-PDIA6 Antibody Products BiocompareAnti-PDIA6 antibodies are offered by a number of suppliers.This target gene encodes the protein 'protein disulfide isomerase family A member 6' in humans and may also be known as ERp5,TXNDC7,protein disulfide-isomerase A6,and ER protein 5.Structurally,the protein is reported to be 48.1 kilodaltons in mass.
Host Rabbit IgGy Antibody Selector Quickly search hundreds of thousands of antibodies available for purchase from VWR by selecting common antibody features like antigen symbol and name,reactivity,clonality,conjugation,host,and other key factors.Antibodies used to identify and locate intracellular and extracellular proteins in common applications such as Western Blot,ELISA Anti-PDIA6 antibody (ab11432) AbcamMay 05,2021·Rabbit polyclonal PDIA6 antibody.Validated in WB,ICC/IF and tested in Human.Cited in 9 publication(s).Immunogen corresponding to synthetic peptide.
Rabbit polyclonal PDIA6 antibody.Validated in WB,IP,IHC and tested in Mouse,Human.Immunogen corresponding to synthetic peptide.Anti-Prostate Specific Acid Phosphatase Recombinant AntibodyAnti-Prostate Specific Acid Phosphatase (PSAP) Recombinant Mouse Monoclonal Antibody (Clone:rACPP/1338) Share Figure 1 Formalin-fixed,paraffin-embedded human Prostate Carcinoma stained with PSAP Mouse Recombinant Monoclonal Antibody (rACPP/1338).
Aug 22,2016·The sections were washed and then incubated with a 10-fold dilution of the primary antibody,anti-PD8,for 2 h at RT.Sections were washed and transferred to a 25-fold dilution of secondary antibody goat anti-rabbit IgG-conjugated to 10Biomolecules Free Full-Text Variation in the PDIA6 exhibits disulfide oxidoreductase The proteins were immobilized on a nitrocellulose membrane and GFP was detected using a rabbit anti-GFP primary antiserum,and an anti-rabbit horseradish peroxidase conjugated secondary antiserum. In Figure 7,the GFP fusions were detected by Western blot using a polyclonal rabbit anti-GFP
The protein encoded by this gene is a regulatory protein involved in mitosis.The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF).Two alternative transcripts have been found,a constitutively expressed transcript and aCategory Polyclonal AntibodiesAnti-Protein Disulfide Isomerase Antibody Products Anti-Protein Disulfide Isomerase Antibody Products Listed below are anti-Protein Disulfide Isomerase antibodies from multiple suppliers.Protein Disulfide Isomerase is a reported alias name for the human gene P4HB,or 'prolyl 4-hydroxylase subunit beta'.
MAPK,anti-phospho-JNK1/2,mouse monoclonal antibodies against caspase 3,Bcl-2,Bax,cytochrome c,caspase 3,cytochrome c,anti-PDIA6,and horseradish peroxidase-linked anti-rabbit or anti-mouse IgG were obtained from Cell Signaling Technology,Inc.(Beverly,MA,USA).Rabbit polyclonal antibodiesDeletion of muscle GRP94 impairs both muscle and body May 30,2012·Figure 1.Musclespecific deletion of GRP94.A) Schematic representation of the GRP94 gene showing the loxP sites flanking exon 1,whose deletion removes 900 bp of DNA.B) Example of PCR assays used to genotype mouse tissues.When the WT allele is amplified with primers a (5' to the gene) and b (within exon 2),it yields a 1320bp product from nondeleted tissues (liver and kidney),but only
Jun 17,2019·Antibodies.The following primary rabbit polyclonal antibodies were used antiPDIR,antiERp57,antiP5,and antiGRP170 (all from ThermoFisher,cat.# PA3007,PA3009,PA3008,and PA527655,respectively).In addition,a rabbit polyclonal antibody to the HAtag (Sigma,cat.ERp46 is reduced by high glucose and regulates insulin Subsequently,cells were incubated with anti-ERp46 polyclonal antibody,followed by incubation with fluoresceine-conjugated secondary antibody.Other primary antibodies used for immunofluorescence single-labeling experiments included the following rabbit polyclonal anti-calreticulin,mouse monoclonal anti-PDI,and goat polyclonal anti-insulin.
Unconjugated Whole IgG Rabbit anti-ERp5 Antibody,Affinity Purified suitable for WB,IHC applications.Visit Bethyl for all your antibody needs.We support COVID-19 (SARS-CoV-2) research and are operating in accordance to CDC and government guidelines.ERp5 Antibody Bethyl Laboratories,Inc.Unconjugated Whole IgG Rabbit anti-ERp5 Antibody,Affinity Purified suitable for WB,IP,IHC applications.Visit Bethyl for all your antibody needs.
Jun 11,2019·After 48 hr,cells were treated with DTT (2.5,5 mM) for 20 min or Menadione (50,100 M) for 1 hr.Lysates were analyzed by non-reducing and reducing SDS-PAGE and Western blotting with anti-human proinsulin antibody (1B24).Frontiers The Arabidopsis Protein Disulfide Isomerase Dec 29,2020·The proteins (10 mg/lane) were electrophoresed on a 10% acrylamide SDS-PAGE gel,were electro-transferred to Amersham Protran nitrocellulose membranes (Amersham) and analyzed using the polyclonal rabbit PDI9-specific antiserum (1:500 dilution; except 1:100 for pollen samples) followed by an anti-rabbit HRP-conjugated secondary antibody at 1
The affinitypurified rabbit antiP5 antibody used in this study was kindly provided by Dr T.Komiya (Nagahama Institute of Bio -Science and Technology,Nagahama,Japan) (6).The rabbit antiPDI polyclonal antibody was prepared as previously described (12).Protein-agarose was G PLUS purchased from Santa Cruz Biotechnology,Inc.(Dallas,TX,GRP94 Antibody - AF0735 - affinity_biosciencesPig(100%),Bovine(88%),Sheep(88%),Rabbit(88%),Dog(88%) Source Rabbit.Clonality Polyclonal.Purification The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).Specificity GRP94 Antibody detects endogenous levels of total GRP94.RRID AB_2834142
Dec 16,2015·The following primary antibodies were purchased to characterize proteins of interest a mouse monoclonal antibody to angiotensin converting enzyme (ACE) was purchased from Santa Cruz Biotechnology (Dallas,Texas,USA) [Cat # Sc-23908]),a rabbit polyclonal anti-protein disulfide isomerase 6 (PDIA6) was purchased from Sigma (Cat # HPA034653) and Human PDIA6(Protein Disulfide Isomerase A6) ELISA Kit Description A Rabbit polyclonal antibody against Human Protein Disulfide Isomerase A6 (PDIA6).This antibody is labeled with HRP.Protein Disulfide Isomerase A6 (PDIA6) Polyclonal Antibody (Human),PE
Mar 01,2013·For double immunostaining,primary antibodies in blocking solution were incubated simultaneously overnight at 4 °C,followed by a 2-h incubation with secondary antibodies.Primary antibodies were rabbit polyclonal anti-Myc (1:600) and mouse monoclonal anti-FLAG M2 (1:500) antibodies from Sigma,and mouse monoclonal anti-PDI antibody (1:400 Images of Rabbit Polyclonal Primary Antibodies Anti PDIA6 imagesAnti-PDIA6 / ERP5 Antibody Rabbit anti-Human Polyclonal The antibody is a rabbit polyclonal antibody raised against PDIA6.It has been selected for its ability to recognize PDIA6 in immunohistochemical staining and western blotting.
Sep 21,2017·The cells were then washed and primary antibodies were applied overnight at a dilution of 1:500 to 1:1000 in blocking solution at 4°C,washed in PBS and incubated with secondary antibodies at a dilution of 1:5000 in PBS at room temperature for 1-2 hr.Coverslips were stained with 500 nM DAPI in PBS for 5 min,washed several times with PBS and Integrative Quantitative Proteomics Unveils Proteostasis Proteomics-based clinical studies represent promising resources for the discovery of novel biomarkers or for unraveling molecular mechanisms underlying particular diseases.Here,we present a discovery study of hepatocellular carcinoma developed on nonfibrotic liver (nfHCC) that combines complementary quantitative iTRAQ-based proteomics and phosphoproteomics approaches.Using both approaches
Proteomics-based clinical studies represent promising resources for the discovery of novel biomarkers or for unraveling molecular mechanisms underlying particular diseases.Here,we present a discovery study of hepatocellular carcinoma developed on nonfibrotic liver (nfHCC) that combines complementary quantitative iTRAQ-based proteomics and phosphoproteomics approaches.Using both approaches LC-MS/MS Analysis of Differentially Expressed Glioblastoma Jun 01,2012·The protein bands were electro transferred to a PVDF membrane (Millipore,Bedford,MA),blocked with 2% (v/v) BSA in TBST (150 m m NaCl,20 m m Tris,0.1% Tween 20,pH 7.4) for 2 h at room temperature,followed by incubation with primary antibody (anti-ATL3,1:1000 (polyclonal rabbit,Abcam); anti-NEGR1,1:1000 (polyclonal rabbit,Abcam); anti
Feb 21,2017·The following primary antibodies were used rabbit monoclonal antibody against phospho-AMPK (Thr172) (40H9,no.2535,Cell Signaling),rabbit polyclonal antibodyMonoclonal Polyclonal Antibodies Bio-RadPrimary antibodies directly bind specific antigens with high specificity and affinity.They can be either monoclonal antibodies,which bind to a specific epitope,or polyclonal antibodies that bind to several epitopes of an antigen.Primary antibodies are predominantly used in immunoassays such as ELISA,western blot,immunohistochemistry,chromatin immunoprecipitation (ChIP),or flow cytometry.
Apr 24,2017·Cells were labelled for 1 h 30 min at room temperature with the indicated antibodies (goat polyclonal anti-uromodulin (0855140,MP Biomedicals,Santa Ana,CA,dilution 1:500); rabbit polyclonal anti-calreticulin (C4606,Sigma,dilution 1:500); mouse monoclonal anti KDEL (ADI-SPA-827,Enzo Life Sciences,Farmingdale,NY,dilution 1:500) followed Mutant uromodulin expression leads to altered homeostasis Apr 24,2017·Pre-cleared lysates were then incubated under rotation for 16 hours at 4°C with 20 l protein G-Sepharose beads pre-conjugated with 6 l of sheep polyclonal anti-uromodulin antibody (T0850B,United States Biological) for co-immunoprecipitation of calnexin and PDI or with 6 l of antiserum to human uromodulin (generous gift from Prof
Sep 16,2010·For assays that used murine serum,the rabbit polyclonal anti-2GPI antibody (100nM) was used.After washing 3 times with PBS/Tween (0.1%),goat antimouse or antirabbit immunoglobulin conjugated to alkaline phosphatase was added (1:1500 dilution; at RT for 1 hour),and samples were read after adding appropriate chromogenic substrate.P5 5 Bioz Ratings For Life-Science ResearchArticle Snippet The samples were detected with anti-HIV-1 integrase antibody (abcam; ab66645),anti-HIV-1 p55 + p24 + p17 antibody (abcam; ab63917),anti-HIV1 p24 [39/5.4A] (abcam; ab9071),and second mouse or rabbit antibody (MBL ,LTD.),and anti-beta actin antibody (HRP conjugated) (abcam),and then visualized using SuperSignal WestPico
Rabbit.Clonality Polyclonal. HSPA5,HYOU,PDIA2,PDIA4,PDIA6,PPIB,SDF2L1,UGT1A1 and very small amounts of ERP29,but not,or at very low levels,CALR nor CANX.Similarity Belongs to the protein disulfide isomerase family. Secondary antibody Goat Anti-Rabbit IgG (H+L) HRP Goat Anti-RabbitPDIA6 (NM_005742) Human Tagged ORF Clone RC201710Chromatograms CHROMATOGRAMS Sequencher program is needed,download here.Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map ACCN NM_005742 ORF Size 1320 bp OTI Disclaimer Due to the inherent nature of this plasmid,standard methods to replicate additional amounts of DNA in E.coli are highly likely to result in mutations and/or rearrangements.
The cells were labeled with PDIA6 Rabbit Polyclonal Antibody (Product # PA3-008) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody,Alexa Fluor&488 conjugate (ProductPDIA6 Antibody - Cat.No.XW-8125 ProSciPDIA6 antibody can be stored at 4C for short term (weeks).Long term storage should be at -20C.As with all antibodies care should be taken to avoid repeated freeze thaw cycles.Antibodies should not be exposed to prolonged high temperatures.
The PDIA6 CRISPR guide RNA sequences were designed by GenScript's proprietary algorithm to target a single locus in the endogenous genome.High-specificity gRNA constructs forPDIA6 Polyclonal antibody ,page8.
Jan 25,2010·Primary antibodies (antiERp57 and antiERp72) were added at a 1:500 dilution [in PBS with 01% (w/v) BSA] for 4 h and then unbound antibody removed by washing three times.Antimouse or antirabbit alkaline phosphataseconjugated secondary antibodiesPlatelets release novel thiol isomerase enzymes which are Jan 25,2010·Primary antibodies (antiERp57 and antiERp72) were added at a 1:500 dilution [in PBS with 01% (w/v) BSA] for 4 h and then unbound antibody removed by washing three times.Antimouse or antirabbit alkaline phosphataseconjugated secondary antibodies
Oct 01,2014·After washing three times with 0.01 m PBS,nonspecific binding was blocked with goat serum for 30 min.Tissues were then incubated with primary antibodies at 4 °C overnight followed by incubation with peroxidase-labeled polymer conjugate to anti-rabbit immunoglobulins (Invitrogen,Carlsbad,CA) for 1 h at 37 °C.Proteomic Analysis of Solid Pseudopapillary Tumor of the Oct 01,2014·The following primary antibodies were used anti-MSI2 rabbit affinity isolated,1:200; anti-DKK4 rabbit polyclonal,1:200; anti-JUP mouse clone 2G9,1:150; anti-ACADL rabbit affinity isolated,1:100; anti-EPHX2 rabbit affinity isolated,1:100; and anti-DAD1 rabbit affinity isolated,1:200.
Oct 01,2014·The following primary antibodies were used anti-MSI2 rabbit affinity isolated,1:200; anti-DKK4 rabbit polyclonal,1:200; anti-JUP mouse clone 2G9,1:150; anti-ACADL rabbit affinity isolated,1:100; anti-EPHX2 rabbit affinity isolated,1:100; and anti-DAD1 rabbit affinity isolated,1:200.Proteomic profiling of rapid non-genomic and concomitant Apr 03,2012·The following secondary antibodies were used goat polyclonal anti-rabbit Cy2 or Cy5-labeled (GE Healthcare,1:2000),goat polyclonal anti-mouse Cy3-labeled (GE Healthcare,1:2000),donkey polyclonal anti-goat HRP (sc-2020,1:5000),goat polyclonal anti-rabbit HRP (Pierce,1:5000).
Source The E.Coli derived 32 kDa recombinant protein contains the Nucleocapsid core protein 1-49 amino acids immunodominant regions.SARS Coronavirus is an enveloped virus containing three outer structural proteins,namely the membrane (M),envelope (E),and spike (S) proteins.Some results are removed in response to a notice of local law requirement.For more information,please see here.
Some results are removed in response to a notice of local law requirement.For more information,please see here.12345NextPDIA6 Human qPCR Primer Pair (NM_005742) HP208826
Primary Antibodies; Secondary Antibodies; Tag Antibodies; ELISA antibodies; Proteins Proteins.Overview; Rabbit Polyclonal Anti-PDIA6 Antibody.100 ul; USD 375.00.Add To Cart.RC238272.PDIA6 (myc-DDK-tagged) - Human protein disulfide isomerase family A,member 6 (PDIA6Some results are removed in response to a notice of local law requirement.For more information,please see here.Previous123456NextSynaptotagmin 11 interacts with components of the RNA May 29,2014·Several antibodies were kindly donated the monoclonal antiFMRP antibody 1C3 (Dr.Mazroui,Université Laval,Québec,Canada); a rabbit polyclonal antiCaBP1/PDIA6 (Dr.Nguyen Van,Universität Göttingen,Germany),a monoclonal antiAgo2 (851) (Dr.Hobman,University of Alberta,Edmonton,Canada),a monoclonal antiEEA1 (Dr.Grunberg
May 29,2014·Several antibodies were kindly donated the monoclonal antiFMRP antibody 1C3 (Dr.Mazroui,Université Laval,Québec,Canada); a rabbit polyclonal antiCaBP1/PDIA6 (Dr.Nguyen Van,Universität Göttingen,Germany),a monoclonal antiAgo2 (851) (Dr.Hobman,University of Alberta,Edmonton,Canada),a monoclonal antiEEA1 (Dr.Grunberg Tumor-associated autoantibodies from mouse breast cancer 2 days ago·Alkaline phosphatase-conjugated goat anti-mouse IgG antibody (diluted 1:2000) was the secondary antibody (Thermo Fisher A16069) and nitroblue tetrazolium chloride/5-bromo
2 days ago·Alkaline phosphatase-conjugated goat anti-mouse IgG antibody (diluted 1:2000) was the secondary antibody (Thermo Fisher A16069) and nitroblue tetrazolium chloride/5-bromoUltrastructural variation and key ER chaperones response Jan 25,2021·Membranes were then incubated with one of the following primary antibodies rabbit polyclonal antibody against AjERP29 (0.464 mg/mL,diluted 1:450 for cells in TBS with 5% non-fat powdered milk and 0.05% Tween-20,prepared by Genscipt,Nanjing,China) and rabbit monoclonal antibody against AjPDIA6 (0.364 mg/mL,diluted 1330 in TBS with 5%
Primary Menu.angiotensin pathway; anti growth hormone; anti his antibody; anti human igd; anti human igg; Human PDIA6(Protein Disulfide Isomerase A6) ELISA Kit; Human PEBP1(Phosphatidylethanolamine Binding Protein 1) ELISA Kit Rabbit Polyclonal antibody Anti-CRBN Anti-CRBN ImmunoStep 50 µg EUR 349 Human Immunoglobulin G (IgG anti-Homo sapiens (Human) ERO1B Antibody raised inRabbit anti-Homo sapiens (Human) ERO1B Polyclonal antibody Uniprot No.Q86YB8 Target Names (50% of P4HB reoxidation rate),as well as PDIA3,PDIA4,PDIA6 and NXNDC12 (10%).Following P4HB reoxidation,passes its electrons to molecular oxygen via FAD,leading to the production of reactive oxygen species (ROS) in the cell.
Lane 1 Rabbit control IgG instead of (1g) instead of CSB-PA010824ESR2HU in Hela whole cell lysate.For western blotting,a HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2 CSB-PA010824ESR2HU (4g) + Hela whole cell lysate (500g) Lane 3 Hela whole cell lysate (20g)anti-PDIA6 antibody Product No.ABIN3002737anti-Protein Disulfide Isomerase Family A,Member 6 (PDIA6) (Middle Region) antibody Primary Antibody.PDIA6 Reactivity Human,Mouse,Rat,Cow,Dog,Guinea Pig,Horse,Pig,Rabbit IHC,WB Host Rabbit Polyclonal unconjugated
Pcr controls and Antibodies.Primary Menu.angiotensin pathway; anti growth hormone; anti his antibody; anti human igd; anti human igg; anti idiotype; anti lps antibody; anti mouse; anti myc antibody; Antikroppar; apoptosis detection kit; aqueous mounting medium; atlanta biologicals fbs;